Genomic and phylogenetic analysis of choriolysins, and biological activity of hatching liquid in the flatfish Senegalese sole
Autoría
Carballo, C., Chronopoulou, E.G., Letsiou, S., Spanidi, E., Gardikis, K., Labrou, N.E. y Manchado, M.
Subtipo
Artículos Científicos
Fecha de publicación
Palabras clave
Solea senegalensis
lenguado
Ámbito
Acuicultura y Pesca
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Resumen
The hatching enzymes or choriolysins are key proteases in fish life cycle controlling the release of larvae to surrounding environment that have been suggested as target for novel biotechnological uses. Due to the large amounts of eggs released by the flatfish Solea senegalensis, during the spawning season, the hatching liquid properties and choriolysin-encoding genes were investigated in this species. A genomic analysis identified four putative genes referred to as SseHCEa, SseHCEb, SseLCE and SseHE. The phylogenetic analysis
classified these paralogs into two clades, the clade I containing SseHCE paralogs and the clade II containing two well-supported subclades named as HE and LCE. The two SseHCE paralogs were intron-less and both genes were tandemly arrayed very close in the genome.
The synteny and gene rearrangement identified in the flatfish lineage indicated that the duplication of these two paralogs occurred recently and they are under divergent evolution.
Citación
PLoS ONE 14(12) (2019) https://doi.org/10.1371/journal. pone.0225666
Editorial
PLOS
ISSN
ISSN 1932-6203 (Online)
DOI
https://doi.org/10.1371/journal.pone.0225666
Participación IFAPA
CARLOS CARBALLO PEREZ
MANUEL MANCHADO CAMPAÑA
Centros
Centro IFAPA El Toruño
Áreas de actuación
Acuicultura y Recursos Marinos
Agencia financiadora
This work was supported by research grant Algae4A&B from the UE H2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 691102. The funders had no role in study design
data collection and analysis
decision to publish
or preparation of the manuscript. APIVITA provided support in the form of salaries for authors KG
ES and SL that participated in the Algae4A&B training activities. These authors contributed to data collection as part of the training secondments but did not have any additional role in the study design
data analysis
decision to publish
or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.
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