Photobacterium toruni sp nov., a bacterium isolated from diseased farmed fish
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Autoría
Labella, Alejandro M.; Arahal, David R.; Lucena, Teresa; Manchado, Manuel; Castro, Dolores; Borrego, Juan J.
Subtipo
Artículos Científicos
Fecha de publicación
Palabras clave
Peces
diseased fish
draft genome
farmed fish
Photobacterium and Vibrionaceae
Ámbito
Acuicultura y Pesca
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Resumen
DOI: 10.1099/ijsem.0.002325
Citación
Labella et al., Int J Syst Evol Microbiol 2017;67:4518–4525
Editorial
IUMS
ISSN
1466-5026
DOI
10.1099/ijsem.0.002325
Participación IFAPA
MANUEL MANCHADO CAMPAÑA
Centros
Centro IFAPA El Toruño
Áreas de actuación
Acuicultura y Recursos Marinos
Abstract
Three bacterial strains were isolated from liver and spleen of diseased farmed redbanded seabream (Pagrus auriga) in south-west Spain. Their partial 16S rRNA gene sequences clustered within those of the genus Photobacterium , showing high similarity (98.6–99.3 %) to the type strains of Photobacterium iliopiscarium , P. piscicola , P. kishitanii , P. aquimaris and P. phosphoreum . Multilocus sequence analysis using six housekeeping genes (gapA, topA, mreB, ftsZ, gyrB and 16S rRNA) confirmed the new strains as forming an independent branch with a bootstrap value of 100, likely to represent a novel species. To confirm this, we used whole genome sequencing and genomic analysis (ANIb, ANIm and in silico DNA–DNA hybridization) obtaining values well below the thresholds for species delineation. In addition, a phenotypic characterization was performed to support the description and differentiation of the novel strains from related taxa. Cells were Gram-stain-negative, motile bacilli, chemo-organotrophic and ...
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