I. In vitro (RCC, PC and MC)
Microfluidics and organoids.
- Collection of tumour tissue samples for preclinical in vitro platforms and collection of clinical data.
- Development of organoid biobank for colorectal, lung and breast cancer.
- Isolation and / or differentiation of immune cells
- Development of microfluidic platform for mixed organoid/stromal cell culture
- Therapeutic strategies in 3D organoid culture and on microfluidic platforms
II. In vivo (CRC):
Humanised murine models.
- Collection of CRC tumour tissue samples for preclinical PDX platforms (in vivo), and collection of clinical data.
- Processing of clinical samples
- Extraction of DNA, RNA and proteins from tumour samples.
- Immunohistochemical (IHC) analysis. Classification of histological subtypes and associated PDX models.
- Development of humanised murine models
- Immune profile analysis fortumours and PDXs.
- Therapeutic strategies in PDX models and humanised PDX models
