I+D+i
La investigación biomédica requiere para su desarrollo muestras biológicas y datos clínicos asociados. Para responder a ello, el Biobanco del SSPA mantiene una constante renovación e innovación de sus procedimientos, desarrollando una I+D+i propia que repercute en una mayor calidad de las muestras y en la mejora de los procedimientos, contribuyendo directamente a un incremento de la calidad de la investigación biomédica.
Actualmente el Biobanco del SSPA tiene varias líneas de I+D+i en Biobancos:
- Sistemas de información para Biobancos y Registro de Donantes.
- Estrategias de sostenibilidad, optimización y mejora de los procedimientos operativos del Biobanco.
- Variables pre analíticas de las muestras biológicas y búsqueda de marcadores de calidad.
- Desarrollo e innovación en procesos de gestión y calidad en biobancos.
Estas líneas son el resultado de:
- Preguntas de investigación o necesidades identificadas en algunas de las áreas de trabajo del Biobanco del SSPA: calidad, comunicación, área tecnológica y preservación.
- Los objetivos comprometidos con las Plataformas Tecnológicas.
- Colaboraciones con empresas, grupos de investigación o grupos de trabajo.
- Desarrollos o validación de productos/protocolos/tecnologías.
- De la participación en ensayos clínicos o consorcios de investigación.
Actuaciones I+D+i ejecutadas a través del Plan de Recuperación, Transformación y Resiliencia.
El Área de Investigación del Biobanco del SSPA participa en el desarrollo del proyecto de investigación “Solución de biopsia líquida para el diagnóstico precoz del cáncer mediante secuenciación de ARN circulante y métodos de aprendizaje automático”, de forma colaborativa con el Centro Tecnológico LEITAT y FLOMICS BIOTECH S.L., en el marco de la actuación con referencia CPP2021-008897 financiada por MCIN/AEI/10.13039/501100011033 y por la Unión Europea “NextGenerationEU”/PRTR, incluida en la resolución de concesión de ayudas a proyectos de colaboración público-privada de la Agencia Estatal de Investigación, de fecha de 23 de diciembre de 2022, del Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia, del Plan Estatal de investigación Científica, Técnica y de Innovación 2021-2023, en el marco del Plan de Recuperación, Transformación y Resiliencia.
Actuaciones I+D+i ejecutadas en el Programa Operativo 2014-2020
En el Nodo Coordinador del Área de Investigación del Biobanco del SSPA se ejecutan actuaciones de I+D+i cofinanciadas tanto por el Fondo Europeo de Desarrollo Regional (FEDER) como por el Fondo Social Europeo (FSE). En el marco del período de programación 2014-2020, y en cumplimiento del anexo XII del Reglamento (CE) 1303/2013, que establece las obligaciones en materia de Información y Comunicación sobre el apoyo procedente de estos fondos, se hace público el listado de actuaciones ejecutadas, al que se puede acceder a través del siguiente enlace, así como información esencial de las mismas.
Publicaciones Científicas del Biobanco del SSPA
Listado de publicaciones derivadas de la actividad científica del Biobanco del SSPA
- Quality control of cell lines using DNA as target. 2022. DNA.
- Morphofunctional and molecular assessment of nutritional status in head and neck cancer patients undergoing systemic treatment: Role of inflammasome in clinical nutrition. 2022. Cancers (Basel).
- The N-terminal subunit of the porcine deltacoronavirus spike recombinant protein (S1) Does not serologically cross-react with other porcine coronaviruses. 2022. Pathogens.
- Novel genes and sex differences in COVID-19 severity. 2022. Human Molecular Genetics.
- Whole-slide image analysis identifies a high content of Hodgkin Reed-Sternberg cells and a low content of T lymphocytes in tumor microenvironment as predictors of adverse outcome in patients with classic Hodgkin lymphoma treated with ABVD. 2022. Frontiers in Oncology.
- Methods for identifying biomedical translation: a systematic review. 2022. American Journal of translational research.
- The Need for Standardization in next-generation sequencing studies for classic Hodgkin Lymphoma: A systematic review. 2022. Diagnostics (Basel).
- Identification of histological threshold concepts in health sciences curricula: Students' perception. 2022. Anatomical sciences educations.
- Selective histone methyltransferase G9a inhibition reduces metastatic development of Ewing sarcoma through the epigenetic regulation of NEU1. 2022. Oncogene.
- The Andalusian Resgistry of Donors for Biomedical Research: Five Years of History. 2021. BioTech.
- Infection and immune response to porcine hemagglutinating encephalomyelitis virus in grower pigs. 2021. Veterinary Microbiology.
- A cytokine network profile delineates a common Th1/Be1 pro-inflammatory group of patients in four systemic autoimmune diseases. 2021. Arthritis Rheumatology.
- Integrative Analysis Reveals a Molecular Stratification of Systemic Autoimmune Diseases. 2021. Arthritis Rheumatology.
- Comparing RNA extraction methods to face the variations in RNA quality using two human biological matrices. 2021. Research Square.
- Porcine Hemagglutinating Encephalomyelitis Virus Infection In Vivo and Ex Vivo. 2021. Journal of virology.
- Integrative epigenomics in Sjögren´s syndrome reveals novel pathways and a strong interaction between the HLA, autoantibodies and the interferon signatura. 2021. Scientific reports.
- A new molecular classification to drive precision treatment strategies in primary Sjögren's syndrome. 2021. Nature comunications.
- Genomic Risk Score impact on susceptibility to systemic sclerosis. 2021. Annals of the rheumatic diseases.
- An elevated polyclonal free light chain level reflects a strong interferon signature in patients with systemic autoimmune diseases. 2021. Journal of translational autoimmunity.
- Metalloproteinases 1 and 3 as Potential Biomarkers in Breast Cancer Development. 2021. International Journal Molecular Science
- Agrodiag PorCoV: a multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses. 2020. Journal of Inmunological Methods.
- Evaluation of RNA purification methods by using different blood stabilization tubes: identification of key features for epidemiological studies. 2020. BMC Medical Research Methodology.
- Impact of different stabilization methods on RT-qPCR results using human lung tissue samples. 2020. Scientific Reports.
- Harmonized and Quality Sample Handling in Biobank-Supported Kulticenter Prospective Studies. 2020. IntechOpen.
- Restoration of MHC-I on tumor cells by fhit transfection promotes immune rejection and acts as an individualized immunotherapeutic vaccine. 2020. Cancers.
- Mutation in ROBO3 Gene in Patients with Horizontal Gaze Palsy with Progressive Scoliosis Syndrome. 2020. International Journal of Enviromental Research and Public Health.
- Low Prevalence of HER2-Positive Breast Carcinomas among Screening Detected Breast Cancers. 2020. Cancers (Basel).
- Corrigendum to "IL8 and EDEM3 gene expression ratio indicates peripheral blood mononuclear cell (PBMC) quality" |LS|Journal of Immunological Methods 465 (2019) 13-19|RS|. 2020. Journal of Immunological Methods.
- Antiphospholipid autoantibody detection is important in all patients with systemic autoimmune diseases. 2020. Journal of Autoimmunity.
- Standardization procedure for flow cytometry data harmonization in prospective multicenter studies. 2020. Scientific Reports.
- IgM antibodies against malondialdehyde and phosphorylcholine in different systemic rheumatic diseases. 2020. Scientific Reports.
- Metabolic Disturbances in Urinary and Plasma Samples from Seven Different Systemic Autoimmune Diseases Detected by HPLC-ESI-QTOF-MS. 2020. Journal Proteome Research.
- Discovering new metabolite alterations in primary sjögren's syndrome in urinary and plasma samples using an HPLC-ESI-QTOF-MS methodology. 2020. Journal of Pharmaceutical and Biomedical Analysis.
- A Case Report of Switching from Specific Vendor-Based to R-Based Pipelines for Untargeted LC-MS Metabolomics. 2020. Metabolites.
- Detecting and Monitoring Porcine Hemagglutinating Encephalomyelitis Virus, an Underresearched Betacoronavirus. 2020. mSphere.
- Biobanking Spotlight on Europe, Middle East, and Africa: Presenting the Collective Experience of the ISBER-EMEA Regional Ambassadors. 2020. Biopreservation and Biobanking.
- Pluripotent stem cell regulation in Spain and the Spanish National Stem Cell Bank. 2020. Stem Cell Research.
- Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming. iScience. 2020. iScience.
- iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migration Stimulation. 2020. Cells.
- Stabilization of Human Whole Blood Samples for Multicenter and Retrospective Immunophenotyping Studies. 2020. Cytometry A.
- An Evolutive and Scientometric Research on Tissue Engineering Review. 2020. Tissue Engineering, Part A.
- Detergent-based decellularized peripheral nerve allografts: An in vivo preclinical study in the rat sciatic nerve injury model. 2020. Journal of Tissue Engineering and Regenerative Medicine.
- Evaluation of myopic cornea lenticules. A histochemical and clinical correlation. 2020. Experimental Eye Research.
- Expanded Differentiation Capability of Human Wharton's Jelly Stem Cells Toward Pluripotency: A Systematic Review. 2020. Tissue Engineering Part B Reviews.
- The challenge of discovering the threshold concepts of medical research areas: A bibliometrics-based approach. 2020. Medical Hypotheses.
- Virus emergentes y nuevas zoonosis. Lecciones aprendidas de una pandemia. 2020. Actualidad Médica.
- In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network. 2019. Journal of Translational Medicine.
- On the Use of Buffy or Whole Blood for Obtaining DNA of High Quality and Functionality: What Is the Best Option?. 2019. Biopreservation and Biobanking
- IL8 and, EDEM3 gene expression ratio indicates peripheral blood mononuclear cell (PBMC) quality. 2019. Journal of Immunological Methods.
- Validation and meta-analysis of kappa index biomarker in multiple sclerosis diagnosis. 2019. Autoimmunity reviews.
- Quality assessment on the long-term cryopreservation and nucleic acids extraction processes implemented in the andalusian public biobank. 2019. Cell Tissue Banking.
- Epigenome-Wide Comparative Study Reveals Key Differences Between Mixed Connective Tissue Disease and Related Systemic Autoimmune Diseases. 2019. Frontiers in Immunology.
- Urinary and plasma metabolite differences detected by HPLC-ESI-QTOF-MS in systemic sclerosis patients. 2019. Journal of Pharmaceutical and Biomedical Analysis.
- Molecular Characterization of Monocyte Subsets Reveals Specific and Distinctive Molecular Signatures Associated With Cardiovascular Disease in Rheumatoid Arthritis. 2019. Frontiers in Immunology
- A new procedure for DNA isolation from saliva samples and comparative analysis of quality indicators. 2019. Journal of Biothecniques
- Effect of continuous positive airway pressure on inflammatory, antioxidant, and depression biomarkers in women with obstructive sleep apnea: a randomized controlled trial. 2019. Sleep.
- Validation and Meta-analysis of kappa index Biomarker in Multiple Sclerosis Diagnosis. 2019. Autoimmunity Reviews.
- Urinary and plasma metabolite differences detected by HPLC-ESI-QTOF-MS in systemic sclerosis patients. 2019
- Identification of the m missing protein hyaluronan synthase 1 in human mesenchymal stem cells derived from adipose tissue or umbilical cord. 2018. Journal Proteome Research.
- Mesenchymal stem cell's secretome promotes selective enrichment of cancer stem-like cells with specific cytogenetic profile. 2018. Cancer Letter.
- Standard PRE analytical Code version 3.0. 2018. Biopreservation and Biobanking.
- IL8 and IL16 levels indicate serum and plasma quality. 2018. Clinical Chemistry and Laboratory Medicine.
- Quality management systems for laboratories and external quality assurance Programs. 2018. IntechOpen.
- Genetic association analysis of vitamin D receptor gene polymorphisms and obesity-related phenotypes. 2018. Gene.
- MHC Intratumoral Heterogeneity May Predict Cancer Progression and Response to Immunotherapy. 2018. Frontiers in Immunology.
- Development of COL3A1-Specific Real-Time PCR Assay for Clinical and Sport Detection Applications. 2018. Journal of Chromatography.
- Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) the Hot Start experience. 2017. Stem Cell Research.
- Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking Cell Tissue. 2017. Cell and Tissue Bank.
- Prevalence and genetic diversity of Trichomonas vaginalis in the general population of Granada and co-infections with Gardnerella vaginalis and Candida species. 2017. Journal of Medical Microbiology.
- DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis. 2016. Biopreservation and Biobanking.
- Points to consider in the development of seed stocks of pluripotent stem cells for clinical applications: International Stem Cell Banking Initiative (ISCBI). 2015. Regenerative Medicine.
- Morphology, cell viability, karyotype, expression of surface markers and plasticity of three human primary cell line cultures before and after the cryostorage in LN2 and GN2. 2015. Cryobiology.