R&D&I
Biomedical research requires biological samples and associated clinical data for its development. To respond to this, the SSPA Biobank hasn has a constant renewal and innovation of its procedures, developing its own R&D that has an impact on a higher quality of samples and on the improvement of procedures, directly contributing to an increase in the quality of biomedical research.
Currently, the SSPA Biobank has several lines of R&D in Biobanks:
- Information systems for Biobanks and Donor Registry.
- Strategies for sustainability, optimization and improvement of the operating procedures of the Biobank.
- Pre-analytical variables of biological samples and search for quality markers.
- Development and innovation in management and quality processes in biobanks.
These lines are the result of:
- Research questions or needs identified in some of the areas of work of the SSPA Biobank: quality, communication, technological area and preservation.
- The objectives committed to the Platformsmore Technological.
- Collaborations with companies, research groups or working groups.
- Development or validation of products/protocols/technologies.
- Participation in clinical trials or research consortia.
R&D&I actions executed through the Recovery, Transformation andResilience.
The Research Area of the SSPA Biobank is involved in the development of the research project "Liquid biopsy solution for early cancer diagnosis through circulating RNA sequencing and machine learning methods," in collaboration with the LEITAT Technology Center and FLOMICS BIOTECH S.L. This is part of the action with reference CPP2021-008897, funded by MCIN/AEI/10.13039/501100011033 and by the European Union "NextGenerationEU"/PRTR. It is included in the grant resolution for public-private collaboration projects issued by the State Research Agency on December 23, 2022, under the State Program to Promote Scientific and Technical Research and its Transfer, from the State Plan for Scientific, Technical, and Innovation Research 2021-2023, within the framework of the Recovery, Transformation, and Resilience Plan.
R&D actions implemented in the 2021-2027 Operational Programme
In the Coordinating Node of the Biobank Research Area of the SSPA, R&D activities co-financed by both the European Regional Development Fund (ERDF) and the European Social Fund (ESF ) are carried out. Within the framework of pprogramming period 2021-2027, and in compliance with Annex XII of Regulation (EC) 1303/2013, which establishes the obligations in terms of Information and Communication on the support from these funds, the list of actions carried out is made public, which can be accessed through the following link, whose file contains essential information about them.
SSPA Biobank Scientific Publications
List of publications derived from the scientific activity of the SSPA Biobank
- Data Model for the Comprehensive Management of Biobanks and Its Contribution to Personalized Medicine. 2024. Journal of Personalized Medicine.
- Not all roads lead to the immune system: The genetic basis of multiple sclerosis severity. 2023. Brain.
- Association of Serum Neurofilament Light Chain Levelst Disease Onset with Disability Worsening in Patients with a First Demyelinating Multiple Sclerosis Event Not Treated with High-Efficacy Drugs. 2023. JAMA Neurology.
- Identification of cell culture contamination by an unusual species of Mycoplasma related to the M. mycoides cluster. 2023. Cytotechnology.
- The pediatric leukemia oncoprotein NUP98-KDM5A induces genomic instability that may facilitate malignant transformation. 2023. Cell Death Discovery.
- Consent and Assent by Children. 2023. GDPR Requirements for Biobanking Activities Across Europe.
- Development and Evaluation of a New qPCR Assay for the Detection of Mycoplasma in Cell Cultures. 2023. Current Issues in Molecular Biology.
- Quality control of cell lines using DNA as target. 2022. DNA.
- Morphofunctional and molecular assessment of nutritional status in head and neck cancer patients undergoing systemic treatment: Role of inflammasome in clinical nutrition. 2022. Cancers (Basel).
- The N-terminal subunit of the porcine deltacoronavirus spike recombinant protein (S1) Does not serologically cross-react with other porcine coronaviruses. 2022. Pathogens.
- Novthe genes and sex differences in COVID-19 severity. 2022. Human Molecular Genetics.
- Whole-slide image analysis identifies a high content of Hodgkin Reed-Sternberg cells and a low content of T lymphocytes in tumor microenvironment as predictors of adverse outcome in patients with CLAssic Hodgkin lymphoma treated with ABVD. 2022. Frontiers in Oncology.
- Methods for identifying biomedical translation: a systematic review. 2022. American Journal of translational research.
- The Need for Standardization in next-generation sequencing studies for classic Hodgkin Lymphoma: A systematic review. 2022. Diagnostics (Basel).
- Identification of histological threshold concepts in health sciences curricula: Students' perception. 2022. Anatomicto sciences educations.
- Selective histone methyltransferase G9a inhibition reduces metastatic development of Ewing sarcoma through the epigenetic regulation of NEU1. 2022. Oncogene.
- The Andalusian Resgistry of Donors for Biomedical Research: Five Years of History. 2021. BioTech.
- Infection and immune response to porcine hemagglutinating encephalomyelitis virus in grower pigs. 2021. Veterinary Microbiology.
- A cytokine network profile delineates a common Th1/Be1 pro-inflammatory group of patients in four systemic autoimmune diseases. 2021. Arthritis Rheumatology.
- Integrative Analysis Reveals a Molecular Stratification of Systemic Autoimmune Diseases. 2021. Arthritis Rheumatology.
- Comparing RNA extraction methods to face the variations in RNA quality using two human biological matrices. 2021. Research Square.
- Porcine Hemagglutinating Encephalomyelitis Virus Infection In Vivo and Ex Vivo. 2021. Journal of virology.
- Integrative epigenomics in Sjögren's syndrome reveals novel pathways and a strong interaction between the HLA, autoantibodies and the interferon signature. 2021. Scientific reports.
- A new molecular classification to drive precision treatment strategies in primary Sjögren's syndrome. 2021. Nature communications.
- Genomic Risk Score impact on susceptibility to systemic sclerosis. 2021. Annals of the rheumatic diseases.
- An elevated polyclonal free light chain level reflects a strong interferon signature in patients with systemic autoimmune diseases. 2021. Journal of translational autoimmunity.
- Metalloproteinases 1 and 3 as Potential Biomarkers in Breast Cancer Development. 2021. International Journal Molecular Science
- Agrodiag PorCoV: a multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses. 2020. Journal of Immunological Methods.
- Evaluationof RNA purification methods by using different blood stabilization tubes: identification of key features for epidemiological studies. 2020. BMC Medical Research Methodology.
- Impact of different stabilization methods on RT-qPCR results using human lung tissue samples. 2020. Sscientitific Reports.
- Harmonized and Quality Sample Handling in Biobank-Supported Kulticenter Prospective Studies. 2020. IntechOpen.
- Restoration of MHC-I on tumor cells by fhit transfection promotes immunerejection and acts as an individualized immunotherapeutic vaccine. 2020. Cancers.
- Mutation in ROBO3 Gene in Patients with Horizontal Gaze Palsy with Progressive Scoliosis Syndrome. 2020. International Journal of Environmental Research and Public Health.
- Low Prevalence of HER2-Positive Breast Carcinomas among Screening Detected Breast Cancers. 2020. Cancers (Basel).
- Corrigendum to "IL8 and EDEM3 gene expression ratio indicates peripheral blood mononuclear cell (PBMC) quality"|LS|Journal of Immunological Methods 465 (2019) 13-19|RS|. 2020. Journal of Immunological Methods.
- Antiphospholipid autoantibody detection is important in all patients with systemic autoimmune diseases. 2020. Journal of Autoimmunity.
- Standardization procedure for flow cytometry data harmonization in prospective multicenter studies. 2020. Scientific Reports.
- IgM antibodies against malondialdehyde and phosphorylcholine in different systemic rheumatic diseases. 2020. Scientific Reports.
- Metabolic Disturbances in Urinary and Plasma Samples from Seven Different Systemic Autoimmune Diseases Detected by HPLC-ESI-QTOF-MS. 2020. Journal Proteome Research.
- Discovering new metabolite alterations in primary sjögren's syndrome in urinary and plasma samples using an HPLC-ESI-QTOF-MS methodology. 2020. Journal of Pharmaceutical and Biomedical Analysis.
- A Case Report of Switching from Specific Vendor-Based to R-Based Pipelines for Untargeted LC-MS Metabolomics. 2020. Metabolites.
- Detecting and Monitoring Porcine Hemagglutinating Encephalomyelitis Virus, an Underresearched Betacoronavirus. 2020. mSphere.
- Biobanking Spotlight on Europe, MiddleEast, and Africa: Presenting the Collective Experience of the ISBER-EMEA Regional Ambassadors. 2020. Biopreservation and Biobanking.
- Pluripotent stem cell regulation in Spain and the Spanish National Stem Cell Bank. 2020. Stem Cell Research.
- Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming. iScience. 2020. iScience.
- iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migratestion Stimulation. 2020. Cells.
- Stabilization of Human Whole Blood Samples for Multicenter and Retrospective Immunophenotyping Studies. 2020. Cytometry A.
- An Evolutionary and Scientometric Research on Tissue Engineering Review. 2020. Tissue Engineering, Part A.
- Detergent-based decellularized peripheral nerve allografts: An in vivo preclinical study in the rat sciatic nerve injury model. 2020. Journal of Tissue Engineering and Regenerative Medicine.
- Evaluation of myopic cornea lenticules. A histochemical and clinical correlation. 2020. Experimental Eye Research.
- Expanded Differentiation Capability of Human Wharton's Jelly Stem Cells Toward Pluripotency: A Systematic Review. 2020. Tissue Engineering Part B Reviews.
- The challenge of discovering the threshold concepts of medical research areas: A bibliometrics-based approach. 2020. Medical Hypotheses.
- Emerging viruses and new zoonoses. Lessons learned from apandemic. 2020. Medical News.
- In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Networkork. 2019. Journal of Translational Medicine.
- On the Use of Buffy or Whole Blood for Obtaining DNA of High Quality and Functionality: What Is the Best Option?. 2019. Biopreservation and Biobanking
- IL8 and, EDEM3 gene expression ratio indicates peripheral blood mononuclear cell (PBMC) quality. 2019. Journal of Immunological Methods.
- Validation and meta-analysis of kappa index biomarker in multiple sclerosis diagnosis. 2019. Autoimmunity reviews.
- Quality assessment on the long-term cryopreservation and nucleic acids extraction processes implemented in the andalusian public biobank. 2019. Cell Tissue Banking.
- Epigenome-Wide Comparative Study Reveals Key Differences Between Mixed Connective Tissue Disease and Related Systemic Autoimmune Diseases. 2019. Frontiers in Immunology.
- Urinary and plasma metabolite differences detected by HPLC-ESI-QTOF-MS in systemic sclerosis patients. 2019. Journal of Pharmaceutical and Biomedical Analysis.
- Molecular Characterization of Monocyte Subsets Reveals Specific and Distinctive Molecular Signatures Associated With Cardiovascular Disease in Rheumatoid Arthritis. 2019. Frontiers in Immunology
- A new procedure for DNA isolation from saliva samples and comparative analysis of quality indicators. 2019. Journal of Biothecniques
- Effect of continuous positive airway pressure on inflammatory, antioxidant, and depression biomarkers in women with obstructive sleep apnea: a randomized controlled trial. 2019. Sleep.
- Validation and Meta-analysis of kappa index Biomarker in Multiple Sclerosis Diagnosis. 2019. Autoimmunity Reviews.
- Urinary and plasma metabolite differences detected by HPLC-ESI-QTOF-MS in systemic sclerosis patients. 2019
- Identification of the m missing protein hyaluronan synthase 1 in human mesenchymal stem cells derived from adipose tissue or umbilical cord. 2018. Journal Proteome Research.
- Mesenchymal stem cell's secretome promotes selective enrichment of cancer stem-like cells with specific cytogenetic profile. 2018. Cancer Letter.
- Standard PRE analytical Code version 3.0. 2018. Biopreservation and Biobanking.
- IL8 and IL16 levels indicate serum and plasma quality. 2018. Clinical Chemistry and Laboratory Medicine.
- Quality management systems for laboratories and external quality assurance Programs. 2018. IntechOpen.
- Genetic association analysis of vitamin D receptor gene polymorphisms and obesity-related phenotypes. 2018. Gene.
- MHC Intratumoral Heterogeneity May Predict Cancer Progression and Response to Immunotherapy. 2018. Frontiers in Immunology.
- Development of COL3A1-Specific Real-Time PCR Assay for Clinical and Sport Detection Applications. 2018. Journal of Chromatography.
- Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) the Hot Start experience. 2017. Stem Cell Research.
- Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking Cell Tissue. 2017. Cell and Tissue Bank.
- Prevalence and genetic diversity of Trichomonas vaginalis in the general population of Granada and co-infections with Gardnerella vaginalis and Candida species. 2017. Journal of Medical Microbiology.
- DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis. 2016. Biopreservation and Biobanking.
- Points to consider in the development of seed stocks of pluripotent stem cells for clinical applications: International Stem Cell Banking Initiative (ISCBI). 2015. Regenerative Medicine.
- Morphology, cell viability, karyotype, expression of surface markers and plasticity of three human primary cell line cultures before and after the cryostorage in LN2 and GN2. 2015. Cryobiology.